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Abstract

The theme of the 1987 Conference of the International Council for Educational Media to be held at Cardiff on the 26‐27 October is ‘Communication ‐‐ the Essence of Education’.  相似文献   
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Progress in microelectrode-based technologies has facilitated the development of sophisticated methods for manipulating and separating cells, bacteria, and other bioparticles. For many of these various applications, the theoretical modeling of the electrical response of compartmentalized particles to an external field is important. In this paper we address the analysis of the interaction between cells immersed in rf fields. We use an integral formulation of the problem derived from a consideration of the charge densities induced at the interfaces of the particle compartments. The numerical solution by a boundary element technique allows characterization of their dielectric properties. Experimental validation of this theoretical model is obtained by investigating two effects: (1) The influence that dipolar “pearl chaining” has on the dielectrophoretic behavior of human T lymphocytes and (2) the frequency variation of the spin and orbital torques of approaching insulinoma β-cells in a rotating field.  相似文献   
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This Special Topic section is on dielectrophoresis, a growing area of widespread interest and relevance to the microfluidics and nanofluidics community.There was a time when the arrival of a telegram from the local post office would foreshadow a step-function change in one’s equilibrium. An internet service provider can now deliver the same effect, as illustrated by an unexpected e-mail from Leslie Yeo inquiring if I would “be interested in guest editing a special issue of Biomicrofluidics on recent advances in dielectrophoresis (DEP).” Flattery directed towards vanity can produce interesting results—which I hope this special issue of Biomicrofluidics demonstrates. The rationale for this special issue is the belief of the journal’s Editors (Dr. Chia Chang and Dr. Leslie Yeo) that dielectrophoresis is a growing area of widespread interest and relevance to the microfluidics and nanofluidics community. Papers, both fundamental and applied, were solicited from the leaders working across this broad interdisciplinary area of research. I was delighted by the positive responses of those whose invited contributions appear in this special issue—efforts certainly not motivated by vanity but through enthusiasm for the subject. Some of those invited to contribute were unable to do so because of other demands on their time. Ongoing advances being made in DEP, especially in its various applications, will surely merit another special issue in the future and hopefully include contributions from those unable to do so now.Two of the papers in this special issue address fundamental aspects of dielectrophoresis (DEP), namely the influences on DEP from electrical double-layers and from particle-particle interactions. Consideration of electrical double layers associated with charged particle surfaces is particularly important for nanoparticles because their effective polarizabilities, associated with field-induced dynamics of the counterions and co-ions in the double layer, can dominate over the intrinsic polarizability of the particle itself. This can influence, for example, to what extent the observation of changes in the DEP crossover frequency (marking the transition between positive and negative DEP) can be relied upon in new immunoassays based on the DEP behavior of functionalized nanoparticles. By considering the electrodynamics of double layers, Basuray et al.1 propose a theory to predict how the DEP crossover frequency will vary as a function of particle size and the ionic strength of the suspending electrolyte. In their paper, Sancho et al.2 derive a theoretical model to describe how particle-particle interactions (e.g., “pearl-chaining”) influence the DEP crossover frequency value. This model also describes well the changes in electrorotation and a newly observed precession effect as particles approach each other under the influence of a rotating field.DEP at the nanoscale is also addressed in contributions from the groups of Ralph Hölzel, Junya Suehiro, and Karan Kaler. Thus, Henning et al.3 describe a new method, based on the measurement of capacitance changes between planar microelectrodes, for the automatic acquisition of the DEP properties of nanoparticles without the need for labeling protocols or visual observations. Suehiro4 describes how DEP can be employed as a bottom-up approach for fabricating nanomaterial-based devices such as a carbon nanotube gas sensor and a ZnO nanowire photosensor. Kaler et al.5 describe how the DEP manipulation of miniscule amounts of polar aqueous samples, a method known as liquid-DEP, can be used for on-chip bioassays, such as nucleic acid analysis, and through parallel sample processing offer the potential for conducting automated multiplexed assays. The use of DEP to selectively trap and separate cells has been investigated over many years, and contributions from the groups of Hywel Morgan, Ana Valero, Masau Washizu, and Gerard Markx describe the latest advances and applications. Thomas et al.6 describe a new automated DEP cell trap design for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population. Recovery of small populations of human osteoblast-like cells with a purity of 100% is demonstrated. A cell-sorting device, based on the opposition of DEP forces that discriminates between cell types according to such properties as their membrane permittivity and cytoplasm conductivity, is described by Valeroet al.7 The versatility of the device is demonstrated by synchronizing a yeast cell culture at a particular phase of the cell cycle. Gel et al.8 describe a DEP-assisted cell trapping method for fusing pairs of cells in an array of micro-orifices. This method produces not only a high yield of viable cell fusants, but also allows for subsequent study of postfusion cell development. Zhu et al.9 describe a DEP-based microfluidic separation system in which dead and active cells can be collected from a given cell suspension, whilst at the same time eluting dormant cells. In the second paper from Gerard Markx’s group, Zhu et al.10 demonstrate that the rate-limiting resuscitation of a colony of dormant bacteria is determined by the diffusion of a resuscitation-promoting factor into the colony interior. This study involved the artificial engineering of different sizes and shapes of bacterial aggregates using DEP forces. Finally, in my own contribution,11 I have attempted to summarize the growing output of DEP publications in terms of their contributions to the theory, technology, and applications of DEP.  相似文献   
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This article analyses the intellectual discourses of adult development and adult learning. It argues that through a process of transformative adult learning individuals can experience the disintegration and reintegration of past and present human growth. The development of adults is considered not in terms of discrete theories but as a collage of theories. The writers analyse and interpret the literature that attempts to study the interaction of individuals in the total context of the inner and outer forces that impinge on their life.

We conclude from a study of the literature that adult development, from a transformative perspective, is more than adjustment to a particular society; it is a qualitative change in how one views the world; it involves tension and struggle that are productive of a new consciousness. This change occurs through a dialectical process that calls for movement through the old style of meaning‐making to a reconstruction of meaning that is a synthesis of the old and the new. It is also concluded that adult learning can foster critical awareness and critical consciousness that can effect a transformation in the way adults see themselves and others. The route to transformative adult development and transformative learning lies in acknowledging contradictions and differences and working through them, as opposed to ignoring or circumventing them.  相似文献   
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一论文集的一般P-C矩阵由论文数据和引文数据两部分组成.基于一般P-C矩阵并引入"等时性"概念,我们创建了关键变量Ck.Ck表示的是不同年份发表的论文在各自发表后第k年的引文数量的平均值.借助Ck可以构建R族科学发展节律指标,定义为引文观察值和引文期望值的比值,用于反映科学发展的节律.R族指标包括4项指标①指标R--被引年视角加三角形引文窗口;②指标R′--被引年视角加平行四边形引文窗口;③指标r--引用年视角加三角形引文窗口;④指标r′--引用年视角加平行四边形引文窗口.以Journal of Documentation为例,本文具体说明了R族序列的计算和R族指标的应用.  相似文献   
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The effects on retention of adjunct questions either placed at the end of a passage or inserted after their respective paragraphs within the passage were examined. The high school subjects were encouraged to review the materials after encountering the questions, a departure from traditional procedures in the field. Both treatments led to superior retention of the previously questioned facts when compared to a passage-only control group, but did not increase retention of previously unquestioned facts. The position of the adjunct questions did not differentially affect performance. The discrepancy of the results from those traditionally reported are interpreted in terms of the procedures employed.  相似文献   
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